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The Isw2 chromatin remodeling complex represses early meiotic genes upon recruitment by Ume6p.

The ISWI class of chromatin remodeling factors exhibits potent chromatin remodeling activities in vitro. However, the in vivo functions of this class of factors are unknown at a molecular level. We have found that S. cerevisiae Isw2 complex represses transcription of early meiotic genes during mitotic growth in a parallel pathway to Rpd3-Sin3 histone deacetylase complex. This repressor function of lsw2 complex is largely dependent upon Ume6p, which recruits the complex to target genes. Nuclease digestion analyses revealed that lsw2 complex establishes nuclease-inaccessible chromatin structure near the Ume6p binding site in vivo. Based on these findings, we propose a model for the mechanism of transcriptional repression by two distinct chromatin remodeling complexes.

Pubmed ID: 11081629


  • Goldmark JP
  • Fazzio TG
  • Estep PW
  • Church GM
  • Tsukiyama T



Publication Data

October 27, 2000

Associated Grants

  • Agency: NIGMS NIH HHS, Id: GM58465-01

Mesh Terms

  • Binding Sites
  • Chromatin
  • DNA, Fungal
  • DNA-Binding Proteins
  • Epistasis, Genetic
  • Gene Expression Regulation, Fungal
  • Genes, Fungal
  • Histone Deacetylases
  • Macromolecular Substances
  • Meiosis
  • Mitosis
  • Models, Genetic
  • Molecular Conformation
  • Mutation
  • Nuclease Protection Assays
  • Promoter Regions, Genetic
  • Protein Binding
  • RNA, Fungal
  • RNA, Messenger
  • Repressor Proteins
  • Response Elements
  • Saccharomyces cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Transcription Factors