• Register
X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

X

Leaving Community

Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.

No
Yes

Homeobox gene Nkx6.1 lies downstream of Nkx2.2 in the major pathway of beta-cell formation in the pancreas.

Most insulin-producing beta-cells in the fetal mouse pancreas arise during the secondary transition, a wave of differentiation starting at embryonic day 13. Here, we show that disruption of homeobox gene Nkx6.1 in mice leads to loss of beta-cell precursors and blocks beta-cell neogenesis specifically during the secondary transition. In contrast, islet development in Nkx6. 1/Nkx2.2 double mutant embryos is identical to Nkx2.2 single mutant islet development: beta-cell precursors survive but fail to differentiate into beta-cells throughout development. Together, these experiments reveal two independently controlled pathways for beta-cell differentiation, and place Nkx6.1 downstream of Nkx2.2 in the major pathway of beta-cell differentiation.

Pubmed ID: 11076772

Authors

  • Sander M
  • Sussel L
  • Conners J
  • Scheel D
  • Kalamaras J
  • Dela Cruz F
  • Schwitzgebel V
  • Hayes-Jordan A
  • German M

Journal

Development (Cambridge, England)

Publication Data

December 2, 2000

Associated Grants

  • Agency: NIDDK NIH HHS, Id: DK21344
  • Agency: NIDDK NIH HHS, Id: DK41822
  • Agency: NINDS NIH HHS, Id: NS10184
  • Agency: NIDDK NIH HHS, Id: R01 DK021344

Mesh Terms

  • Animals
  • Cell Differentiation
  • Gene Expression Regulation, Developmental
  • Genes, Homeobox
  • Homeodomain Proteins
  • Islets of Langerhans
  • Mice
  • Mice, Knockout
  • Mutation
  • Pancreas
  • Transcription Factors