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LC3, a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing.

The EMBO journal | Nov 1, 2000

http://www.ncbi.nlm.nih.gov/pubmed/11060023

Little is known about the protein constituents of autophagosome membranes in mammalian cells. Here we demonstrate that the rat microtubule-associated protein 1 light chain 3 (LC3), a homologue of Apg8p essential for autophagy in yeast, is associated to the autophagosome membranes after processing. Two forms of LC3, called LC3-I and -II, were produced post-translationally in various cells. LC3-I is cytosolic, whereas LC3-II is membrane bound. The autophagic vacuole fraction prepared from starved rat liver was enriched with LC3-II. Immunoelectron microscopy on LC3 revealed specific labelling of autophagosome membranes in addition to the cytoplasmic labelling. LC3-II was present both inside and outside of autophagosomes. Mutational analyses suggest that LC3-I is formed by the removal of the C-terminal 22 amino acids from newly synthesized LC3, followed by the conversion of a fraction of LC3-I into LC3-II. The amount of LC3-II is correlated with the extent of autophagosome formation. LC3-II is the first mammalian protein identified that specifically associates with autophagosome membranes.

Pubmed ID: 11060023 RIS Download

Mesh terms: Amino Acid Sequence | Animals | Base Sequence | DNA Primers | Fungal Proteins | HeLa Cells | Humans | Intracellular Membranes | Microscopy, Immunoelectron | Microtubule-Associated Proteins | Molecular Sequence Data | Phagosomes | Protein Processing, Post-Translational | Rats | Sequence Homology, Amino Acid | Subcellular Fractions | Transfection

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