Preparing your results

Our searching services are busy right now. Your search will reload in five seconds.

X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

A Drosophila IkappaB kinase complex required for Relish cleavage and antibacterial immunity.

Genes & development | Oct 1, 2000

http://www.ncbi.nlm.nih.gov/pubmed/11018014

Here we report the identification of a Drosophila IkappaB kinase complex containing DmIKKbeta and DmIKKgamma, homologs of the human IKKbeta and IKKgamma proteins. We show that this complex is required for the signal-dependent cleavage of Relish, a member of the Rel family of transcriptional activator proteins, and for the activation of antibacterial immune response genes. In addition, we find that the activated DmIKK complex, as well as recombinant DmIKKbeta, can phosphorylate Relish in vitro. Thus, we propose that the Drosophila IkappaB kinase complex functions, at least in part, by inducing the proteolytic cleavage of Relish. The N terminus of Relish then translocates to the nucleus and activates the transcription of antibacterial immune response genes. Remarkably, this Drosophila IkappaB kinase complex is not required for the activation of the Rel proteins Dif and Dorsal through the Toll signaling pathway, which is essential for antifungal immunity and dorsoventral patterning during early development. Thus, a yet to be identified IkappaB kinase complex must be required for Rel protein activation via the Toll signaling pathway.

Pubmed ID: 11018014 RIS Download

Mesh terms: Animals | Anti-Infective Agents | Drosophila Proteins | Drosophila melanogaster | Gene Expression Regulation | Genes, Insect | I-kappa B Kinase | Insect Proteins | Lipopolysaccharides | Membrane Glycoproteins | Peptides | Phosphorylation | Protein Processing, Post-Translational | Protein Sorting Signals | Protein Subunits | Protein-Serine-Threonine Kinases | RNA, Double-Stranded | Receptors, Cell Surface | Signal Transduction | Toll-Like Receptors | Transcription Factors

Research resources used in this publication

None found

Research tools detected in this publication

None found

Data used in this publication

None found

Associated grants

  • Agency: NIGMS NIH HHS, Id: GM29379
  • Agency: NIGMS NIH HHS, Id: GM59919

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.