• Register
X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

X

Leaving Community

Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.

No
Yes

Cloning of a type I cytokine receptor most related to the IL-2 receptor beta chain.

We have identified a type I cytokine receptor, which we have termed novel interleukin receptor (NILR), that is most related to the IL-2 receptor beta chain (IL-2Rbeta) and physically adjacent to the IL-4 receptor alpha chain gene on chromosome 16. NILR mRNA is most highly expressed in thymus and spleen, and is induced by phytohemagglutinin in human peripheral blood mononuclear cells. NILR protein was detected on human T cell lymphotropic virus type I-transformed T cell lines, Raji B cells, and YT natural killer-like cells. Artificial homodimerization of the NILR cytoplasmic domain confers proliferation to Ba/F3 murine pro-B cells but not to 32D myeloid progenitor cells or CTLL-2 murine helper T cells. In these latter cells, heterodimerization of IL-2Rbeta and the common cytokine receptor gamma chain (gamma(c)) cytoplasmic domains allows potent proliferation, whereas such heterodimerization of NILR with gamma(c) does not. This finding suggests that NILR has signaling potential but that a full understanding of its signaling partner(s) is not yet clear. Like IL-2Rbeta, NILR associates with Jak1 and mediates Stat5 activation.

Pubmed ID: 11016959

Authors

  • Ozaki K
  • Kikly K
  • Michalovich D
  • Young PR
  • Leonard WJ

Journal

Proceedings of the National Academy of Sciences of the United States of America

Publication Data

October 10, 2000

Associated Grants

None

Mesh Terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blotting, Western
  • Cell Line
  • Chromosomes, Artificial, Bacterial
  • Cloning, Molecular
  • DNA, Complementary
  • Humans
  • Interleukin-21 Receptor alpha Subunit
  • Mice
  • Molecular Sequence Data
  • Open Reading Frames
  • Precipitin Tests
  • Receptors, Interleukin-2
  • Receptors, Interleukin-21
  • Sequence Homology, Amino Acid