Preparing your results

Our searching services are busy right now. Your search will reload in five seconds.

X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Class switch recombination and hypermutation require activation-induced cytidine deaminase (AID), a potential RNA editing enzyme.

Cell | Sep 1, 2000

http://www.ncbi.nlm.nih.gov/pubmed/11007474

Induced overexpression of AID in CH12F3-2 B lymphoma cells augmented class switching from IgM to IgA without cytokine stimulation. AID deficiency caused a complete defect in class switching and showed a hyper-IgM phenotype with enlarged germinal centers containing strongly activated B cells before or after immunization. AID-/- spleen cells stimulated in vitro with LPS and cytokines failed to undergo class switch recombination although they expressed germline transcripts. Immunization of AID-/- chimera with 4-hydroxy-3-nitrophenylacetyl (NP) chicken gamma-globulin induced neither accumulation of mutations in the NP-specific variable region gene nor class switching. These results suggest that AID may be involved in regulation or catalysis of the DNA modification step of both class switching and somatic hypermutation.

Pubmed ID: 11007474 RIS Download

Mesh terms: Animals | B-Lymphocytes | Chimera | Cytidine Deaminase | Cytokines | Enzyme Induction | Female | Flow Cytometry | Gene Deletion | Germ-Line Mutation | Germinal Center | Haptens | Heterozygote | Immunoglobulin A | Immunoglobulin Class Switching | Immunoglobulin M | Immunohistochemistry | Lipopolysaccharides | Lymphocyte Activation | Male | Mice | Mice, Knockout | Mutation | RNA Editing | RNA, Messenger | Recombination, Genetic | Spleen | Tumor Cells, Cultured | gamma-Globulins

Research resources used in this publication

None found

Research tools detected in this publication

None found

Data used in this publication

None found

Associated grants

None

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.