• Register
X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

X

Leaving Community

Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.

No
Yes

FATZ, a filamin-, actinin-, and telethonin-binding protein of the Z-disc of skeletal muscle.

We report the identification and characterization of a novel 32-kDa protein expressed in skeletal muscle and located in the Z-disc of the sarcomere. We found that this protein binds to three other Z-disc proteins; therefore, we have named it FATZ, gamma-filamin/ABP-L, alpha-actinin and telethonin binding protein of the Z-disc. From yeast two-hybrid experiments we are able to show that the SR3-SR4 domains of alpha-actinin 2 are required to bind the COOH-terminal region of the FATZ as does gamma-filamin/ABP-L. Furthermore, by using a glutathione S-transferase overlay assay we find that FATZ also binds telethonin. The level of FATZ protein in muscle cells increases during differentiation, being clearly detectable before the onset of myosin. Although FATZ has no known interaction domains, it would appear to be involved in a complex network of interactions with other Z-band components. On the basis of the information known about its binding partners, we could envisage a central role for FATZ in the myofibrillogenesis. After screening our muscle expressed sequence tag data base and the public expressed sequence tag data bases, we were able to assemble two other muscle transcripts that show a high level of identity with FATZ in two different domains. Therefore, FATZ may be the first member of a small family of novel muscle proteins.

Pubmed ID: 10984498

Authors

  • Faulkner G
  • Pallavicini A
  • Comelli A
  • Salamon M
  • Bortoletto G
  • Ievolella C
  • Trevisan S
  • Kojic' S
  • Dalla Vecchia F
  • Laveder P
  • Valle G
  • Lanfranchi G

Journal

The Journal of biological chemistry

Publication Data

December 29, 2000

Associated Grants

  • Agency: Telethon, Id: 1023
  • Agency: Telethon, Id: B.57

Mesh Terms

  • Actinin
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Carrier Proteins
  • Cell Differentiation
  • Cells, Cultured
  • Cloning, Molecular
  • Connectin
  • Contractile Proteins
  • Filamins
  • Humans
  • Mice
  • Microfilament Proteins
  • Molecular Sequence Data
  • Muscle Proteins
  • Muscle, Skeletal