Hot spots in beta-catenin for interactions with LEF-1, conductin and APC.
Interactions between beta-catenin and LEF-1/TCF, APC and conductin/axin are essential for wnt-controlled stabilization of beta-catenin and transcriptional activation. The wnt signal transduction pathway is important in both embryonic development and tumor progression. We identify here amino acid residues in beta-catenin that distinctly affect its binding to LEF-1/TCF, APC and conductin. These residues form separate surface clusters, termed hot spots, along the armadillo superhelix of beta-catenin. We also show that complementary charged and hydrophobic amino acids are required for formation of the bipartite beta-catenin-LEF-1 transcription factor. Moreover, we demonstrate that conductin/axin binding to beta-catenin is essential for beta-catenin degradation, and that APC acts as a cofactor of conductin/axin in this process. Binding of APC to conductin/axin activates the latter and occurs between their SAMP and RGS domains, respectively.
Pubmed ID: 10966653 RIS Download
Adenomatous Polyposis Coli Protein | Amino Acid Sequence | Animals | Axin Protein | Binding Sites | Cell Line | Conserved Sequence | Crystallography, X-Ray | Cytoskeletal Proteins | DNA-Binding Proteins | Dogs | Humans | Ligands | Lymphoid Enhancer-Binding Factor 1 | Models, Molecular | Molecular Sequence Data | Mutation | Neoplasm Proteins | Phosphorylation | Protein Binding | Protein Structure, Secondary | Protein Structure, Tertiary | Trans-Activators | Transcription Factors | Transcriptional Activation | Transfection | Two-Hybrid System Techniques | beta Catenin