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BRCA1 is associated with a human SWI/SNF-related complex: linking chromatin remodeling to breast cancer.

Germline mutations in the tumor suppressor gene, BRCA1, predispose individuals to breast and ovarian cancers. Using a combination of affinity- and conventional chromatographic techniques, we have isolated a predominant form of a multiprotein BRCA1-containing complex from human cells displaying chromatin-remodeling activity. Mass spectrometric sequencing of components of this complex indicated that BRCA1 is associated with a SWI/SNF-related complex. We show that BRCA1 can directly interact with the BRG1 subunit of the SWI/SNF complex. Moreover, p53-mediated stimulation of transcription by BRCA1 was completely abrogated by either a dominant-negative mutant of BRG1 or the cancer-causing deletion in exon 11 of BRCA1. These findings reveal a direct function for BRCA1 in transcriptional control through modulation of chromatin structure.

Pubmed ID: 10943845


  • Bochar DA
  • Wang L
  • Beniya H
  • Kinev A
  • Xue Y
  • Lane WS
  • Wang W
  • Kashanchi F
  • Shiekhattar R



Publication Data

July 21, 2000

Associated Grants


Mesh Terms

  • BRCA1 Protein
  • Breast Neoplasms
  • Chromatin
  • Chromatography, Affinity
  • Chromosomal Proteins, Non-Histone
  • DNA Helicases
  • DNA-Binding Proteins
  • Female
  • HeLa Cells
  • Humans
  • Nuclear Proteins
  • Transcription Factors
  • Transcriptional Activation