Preparing your results

Our searching services are busy right now. Your search will reload in five seconds.

X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

BRCA1 is associated with a human SWI/SNF-related complex: linking chromatin remodeling to breast cancer.

Cell | Jul 21, 2000

http://www.ncbi.nlm.nih.gov/pubmed/10943845

Germline mutations in the tumor suppressor gene, BRCA1, predispose individuals to breast and ovarian cancers. Using a combination of affinity- and conventional chromatographic techniques, we have isolated a predominant form of a multiprotein BRCA1-containing complex from human cells displaying chromatin-remodeling activity. Mass spectrometric sequencing of components of this complex indicated that BRCA1 is associated with a SWI/SNF-related complex. We show that BRCA1 can directly interact with the BRG1 subunit of the SWI/SNF complex. Moreover, p53-mediated stimulation of transcription by BRCA1 was completely abrogated by either a dominant-negative mutant of BRG1 or the cancer-causing deletion in exon 11 of BRCA1. These findings reveal a direct function for BRCA1 in transcriptional control through modulation of chromatin structure.

Pubmed ID: 10943845 RIS Download

Mesh terms: BRCA1 Protein | Breast Neoplasms | Chromatin | Chromatography, Affinity | Chromosomal Proteins, Non-Histone | DNA Helicases | DNA-Binding Proteins | Female | HeLa Cells | Humans | Nuclear Proteins | Transcription Factors | Transcriptional Activation

Research resources used in this publication

None found

Research tools detected in this publication

None found

Data used in this publication

None found

Associated grants

None

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.