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The yeast hnRNP-like protein Hrp1/Nab4 marks a transcript for nonsense-mediated mRNA decay.

Molecular cell | Mar 17, 2000

http://www.ncbi.nlm.nih.gov/pubmed/10882134

The nonsense-mediated mRNA decay (NMD) pathway monitors premature translation termination and degrades aberrant mRNAs. In yeast, it has been proposed that a surveillance complex searches 3' of a nonsense codon for a downstream sequence element (DSE) associated with RNA-binding proteins. An interaction between the complex and the DSE-binding protein(s) triggers NMD. Here we describe the identification and characterization of the Hrp1/Nab4 protein as a DSE-binding factor that activates NMD. Mutations in HRP1 stabilize nonsense-containing transcripts without affecting the decay of wild-type mRNAs. Hrp1p binds specifically to a DSE-containing RNA and interacts with Upf1p, a component of the surveillance complex. A mutation in HRP1 that stabilizes nonsense-containing mRNAs abolishes its affinity for the DSE and fails to interact with Upf1p. We present a model describing how Hrp1p marks a transcript for rapid decay.

Pubmed ID: 10882134 RIS Download

Mesh terms: Biological Transport | Cell Nucleus | Codon, Nonsense | Cytoplasm | Fungal Proteins | Heterogeneous-Nuclear Ribonucleoproteins | Models, Genetic | Mutation | Nuclear Proteins | Phosphoglycerate Kinase | Protein Binding | Protein Biosynthesis | RNA Helicases | RNA Stability | RNA, Fungal | RNA, Messenger | RNA-Binding Proteins | Ribonucleoproteins | Saccharomyces cerevisiae | Saccharomyces cerevisiae Proteins | Untranslated Regions | mRNA Cleavage and Polyadenylation Factors