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The yeast hnRNP-like protein Hrp1/Nab4 marks a transcript for nonsense-mediated mRNA decay.

The nonsense-mediated mRNA decay (NMD) pathway monitors premature translation termination and degrades aberrant mRNAs. In yeast, it has been proposed that a surveillance complex searches 3' of a nonsense codon for a downstream sequence element (DSE) associated with RNA-binding proteins. An interaction between the complex and the DSE-binding protein(s) triggers NMD. Here we describe the identification and characterization of the Hrp1/Nab4 protein as a DSE-binding factor that activates NMD. Mutations in HRP1 stabilize nonsense-containing transcripts without affecting the decay of wild-type mRNAs. Hrp1p binds specifically to a DSE-containing RNA and interacts with Upf1p, a component of the surveillance complex. A mutation in HRP1 that stabilizes nonsense-containing mRNAs abolishes its affinity for the DSE and fails to interact with Upf1p. We present a model describing how Hrp1p marks a transcript for rapid decay.

Pubmed ID: 10882134


  • González CI
  • Ruiz-EchevarrĂ­a MJ
  • Vasudevan S
  • Henry MF
  • Peltz SW


Molecular cell

Publication Data

March 17, 2000

Associated Grants

  • Agency: NIGMS NIH HHS, Id: GM48631

Mesh Terms

  • Biological Transport
  • Cell Nucleus
  • Codon, Nonsense
  • Cytoplasm
  • Fungal Proteins
  • Heterogeneous-Nuclear Ribonucleoproteins
  • Models, Genetic
  • Mutation
  • Nuclear Proteins
  • Phosphoglycerate Kinase
  • Protein Binding
  • Protein Biosynthesis
  • RNA Helicases
  • RNA Stability
  • RNA, Fungal
  • RNA, Messenger
  • RNA-Binding Proteins
  • Ribonucleoproteins
  • Saccharomyces cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Untranslated Regions
  • mRNA Cleavage and Polyadenylation Factors