Two RING finger proteins mediate cooperation between ubiquitin-conjugating enzymes in DNA repair.
Two ubiquitin-conjugating enzymes, RAD6 and the heteromeric UBC13-MMS2 complex, have been implicated in post-replicative DNA damage repair in yeast. Here we provide a mechanistic basis for cooperation between the two enzymes. We show that two chromatin-associated RING finger proteins, RAD18 and RAD5, play a central role in mediating physical contacts between the members of the RAD6 pathway. RAD5 recruits the UBC13-MMS2 complex to DNA by means of its RING finger domain. Moreover, RAD5 association with RAD18 brings UBC13-MMS2 into contact with the RAD6-RAD18 complex. Interaction between the two RING finger proteins thus promotes the formation of a heteromeric complex in which the two distinct ubiquitin-conjugating activities of RAD6 and UBC13-MMS2 can be closely coordinated. Surprisingly, UBC13 and MMS2 are largely cytosolic proteins, but DNA damage triggers their redistribution to the nucleus. These findings suggest a mechanism by which the activity of this DNA repair pathway could be regulated.
Pubmed ID: 10880451 RIS Download
Adenosine Triphosphatases | Biopolymers | Cell Nucleus | Chromatin | Cytoplasm | DNA Damage | DNA Helicases | DNA Repair | DNA-Binding Proteins | Fungal Proteins | Ligases | Protein Binding | Saccharomyces cerevisiae | Saccharomyces cerevisiae Proteins | Ubiquitin-Conjugating Enzymes | Ubiquitin-Protein Ligases