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Requirement of Mis6 centromere connector for localizing a CENP-A-like protein in fission yeast.

Mammalian kinetochores contain the centromere-specific histone H3 variant CENP-A, whose incorporation into limited chromosomal regions may be important for centromere function and chromosome segregation during mitosis. However, regulation of CENP-A localization and its role have not been clear. Here we report that the fission yeast homolog SpCENP-A is essential for establishing centromere chromatin associated with equal chromosome segregation. SpCENP-A binding to the nonrepetitious inner centromeres depended on Mis6, an essential centromere connector protein acting during G1-S phase of the cell cycle. Mis6 is likely required for recruiting SpCENP-A to form proper connection of sister centromeres.

Pubmed ID: 10864871


  • Takahashi K
  • Chen ES
  • Yanagida M


Science (New York, N.Y.)

Publication Data

June 23, 2000

Associated Grants


Mesh Terms

  • Amino Acid Sequence
  • Cell Cycle
  • Cell Cycle Proteins
  • Centromere
  • Chromatin
  • Chromosomal Proteins, Non-Histone
  • Chromosome Segregation
  • DNA Replication
  • DNA, Fungal
  • Fungal Proteins
  • G1 Phase
  • Histones
  • Mitosis
  • Molecular Sequence Data
  • Mutation
  • Recombinant Fusion Proteins
  • S Phase
  • Schizosaccharomyces
  • Schizosaccharomyces pombe Proteins
  • Temperature