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Transcriptional repression by blimp-1 (PRDI-BF1) involves recruitment of histone deacetylase.

B-lymphocyte-induced maturation protein (Blimp-1) is a transcriptional repressor that is considered to be a master regulator of terminal B-cell development because it is sufficient to trigger differentiation in the BCL(1)-cell model. Transcription of the c-myc gene is repressed by Blimp-1 during B-cell differentiation. In this study, we have explored the mechanism by which Blimp-1 represses transcription by using Gal4-fusion protein assays and assays in which Blimp-1 represses the natural c-myc promoter. The results show that Blimp-1 represses the c-myc promoter by an active mechanism that is independent of the adjacently bound activator YY1. Blimp-1 contains two regions that independently associate with histone deacetylase (HDAC) and endogenous Blimp-1 in nuclear extracts binds in vitro to the c-myc Blimp-1 site in a complex containing HDAC. The functional importance of recruiting HDAC for Blimp-1-dependent repression of c-myc transcription is supported by two experiments. First, the HDAC inhibitor tricostatin A inhibits Blimp-1-dependent repression in cotransfection assays. Second, a chromatin immunoprecipitation assay shows that expression of Blimp-1 causes deacetylation of histone H3 associated with the c-myc promoter, and this deacetylation depends on the Blimp-1 binding site in the c-myc promoter.

Pubmed ID: 10713181


  • Yu J
  • Angelin-Duclos C
  • Greenwood J
  • Liao J
  • Calame K


Molecular and cellular biology

Publication Data

April 3, 2000

Associated Grants

  • Agency: NIAID NIH HHS, Id: R01 AI43576

Mesh Terms

  • Acetylation
  • Cell Line
  • Chromatin
  • DNA-Binding Proteins
  • Enzyme Inhibitors
  • Erythroid-Specific DNA-Binding Factors
  • Fungal Proteins
  • Gene Expression Regulation
  • Genes, Reporter
  • Histone Deacetylases
  • Histones
  • Humans
  • Hydroxamic Acids
  • Precipitin Tests
  • Promoter Regions, Genetic
  • Proto-Oncogene Proteins c-myc
  • Recombinant Fusion Proteins
  • Repressor Proteins
  • Saccharomyces cerevisiae Proteins
  • Transcription Factors
  • Transfection
  • YY1 Transcription Factor