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Crystal structure of a fibrillarin homologue from Methanococcus jannaschii, a hyperthermophile, at 1.6 A resolution.

Fibrillarin is a phylogenetically conserved protein essential for efficient processing of pre-rRNA through its association with a class of small nucleolar RNAs during ribosomal biogenesis. The protein is the antigen for the autoimmune disease scleroderma. Here we report the crystal structure of the fibrillarin homologue from Methanococcus jannaschii, a hyperthermophile, at 1.6 A resolution. The structure consists of two domains, with a novel fold in the N-terminal region and a methyltransferase-like domain in the C-terminal region. Mapping temperature-sensitive mutations found in yeast fibrillarin Nop1 to the Methanococcus homologue structure reveals that many of the mutations cluster in the core of the methyltransferase-like domain.

Pubmed ID: 10654930

Authors

  • Wang H
  • Boisvert D
  • Kim KK
  • Kim R
  • Kim SH

Journal

The EMBO journal

Publication Data

February 1, 2000

Associated Grants

None

Mesh Terms

  • Amino Acid Sequence
  • Antigens, Bacterial
  • Bacterial Proteins
  • Chromosomal Proteins, Non-Histone
  • Crystallography, X-Ray
  • Fungal Proteins
  • Methanococcus
  • Methyltransferases
  • Models, Molecular
  • Molecular Sequence Data
  • Mutation
  • Nuclear Proteins
  • Protein Structure, Secondary
  • Protein Structure, Tertiary
  • Recombinant Proteins
  • Ribonucleoproteins, Small Nucleolar
  • Saccharomyces cerevisiae Proteins
  • Sequence Alignment