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Saccharomyces cerevisiae Arc35p works through two genetically separable calmodulin functions to regulate the actin and tubulin cytoskeletons.

Journal of cell science | Feb 24, 2000

Analysis of the arc35-1 mutant has revealed previously that this component of the Arp2/3 complex is involved in organization of the actin cytoskeleton. Further characterization uncovered a cell division cycle phenotype with arrest as large-budded cells. Cells with correctly positioned metaphase spindles accumulated at the restrictive temperature. The observed metaphase arrest most likely occurs by activation of the spindle assembly checkpoint, because arc35-1 was synthetically lethal with a deletion of BUB2. Arc35p activity is required late in G(1) for its cell cycle function. Both the actin and microtubule defects of arc35-1 can be suppressed by overexpression of calmodulin. Analysis of a collection of ts cmd1 mutants for their ability to suppress the actin and/or microtubule defect revealed that the two defects observed in arc35-1 are genetically separable. These data suggest that the actin defect is probably not the cause of the microtubule defect.

Pubmed ID: 10639338 RIS Download

Mesh terms: Actin-Related Protein 2 | Actin-Related Protein 3 | Actins | Alleles | Bridged Bicyclo Compounds, Heterocyclic | Calmodulin | Cell Cycle Proteins | Cell Division | Cytoskeletal Proteins | Cytoskeleton | Fungal Proteins | G1 Phase | Gene Expression Regulation, Fungal | Macromolecular Substances | Metaphase | Microtubules | Saccharomyces cerevisiae | Saccharomyces cerevisiae Proteins | Temperature | Thiazoles | Thiazolidines | Tubulin

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