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Saccharomyces cerevisiae Arc35p works through two genetically separable calmodulin functions to regulate the actin and tubulin cytoskeletons.

Analysis of the arc35-1 mutant has revealed previously that this component of the Arp2/3 complex is involved in organization of the actin cytoskeleton. Further characterization uncovered a cell division cycle phenotype with arrest as large-budded cells. Cells with correctly positioned metaphase spindles accumulated at the restrictive temperature. The observed metaphase arrest most likely occurs by activation of the spindle assembly checkpoint, because arc35-1 was synthetically lethal with a deletion of BUB2. Arc35p activity is required late in G(1) for its cell cycle function. Both the actin and microtubule defects of arc35-1 can be suppressed by overexpression of calmodulin. Analysis of a collection of ts cmd1 mutants for their ability to suppress the actin and/or microtubule defect revealed that the two defects observed in arc35-1 are genetically separable. These data suggest that the actin defect is probably not the cause of the microtubule defect.

Pubmed ID: 10639338


  • Schaerer-Brodbeck C
  • Riezman H


Journal of cell science

Publication Data

February 24, 2000

Associated Grants


Mesh Terms

  • Actin-Related Protein 2
  • Actin-Related Protein 3
  • Actins
  • Alleles
  • Bicyclo Compounds, Heterocyclic
  • Calmodulin
  • Cell Cycle Proteins
  • Cell Division
  • Cytoskeletal Proteins
  • Cytoskeleton
  • Fungal Proteins
  • G1 Phase
  • Gene Expression Regulation, Fungal
  • Macromolecular Substances
  • Metaphase
  • Microtubules
  • Saccharomyces cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Temperature
  • Thiazoles
  • Thiazolidines
  • Tubulin