Our hosting provider is investigating network issues. We apologize for the inconvenience.

Searching across hundreds of databases

Our searching services are busy right now. Your search will reload in five seconds.

X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Saccharomyces cerevisiae Arc35p works through two genetically separable calmodulin functions to regulate the actin and tubulin cytoskeletons.

Journal of cell science | Feb 24, 2000

Analysis of the arc35-1 mutant has revealed previously that this component of the Arp2/3 complex is involved in organization of the actin cytoskeleton. Further characterization uncovered a cell division cycle phenotype with arrest as large-budded cells. Cells with correctly positioned metaphase spindles accumulated at the restrictive temperature. The observed metaphase arrest most likely occurs by activation of the spindle assembly checkpoint, because arc35-1 was synthetically lethal with a deletion of BUB2. Arc35p activity is required late in G(1) for its cell cycle function. Both the actin and microtubule defects of arc35-1 can be suppressed by overexpression of calmodulin. Analysis of a collection of ts cmd1 mutants for their ability to suppress the actin and/or microtubule defect revealed that the two defects observed in arc35-1 are genetically separable. These data suggest that the actin defect is probably not the cause of the microtubule defect.

Pubmed ID: 10639338 RIS Download

Mesh terms: Actin-Related Protein 2 | Actin-Related Protein 3 | Actins | Alleles | Bridged Bicyclo Compounds, Heterocyclic | Calmodulin | Cell Cycle Proteins | Cell Division | Cytoskeletal Proteins | Cytoskeleton | Fungal Proteins | G1 Phase | Gene Expression Regulation, Fungal | Macromolecular Substances | Metaphase | Microtubules | Saccharomyces cerevisiae | Saccharomyces cerevisiae Proteins | Temperature | Thiazoles | Thiazolidines | Tubulin

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.

We have not found any resources mentioned in this publication.