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Calumenin interacts with serum amyloid P component.

We recently reported the identification of human calumenin, a novel Ca(2+) binding, transformation-sensitive and secreted protein [Vorum et al. (1998) Biochim. Biophys. Acta 1386, 121-131; Vorum et al. (1999) Exp. Cell Res. 248, 473-481] belonging to the family of multiple EF-hand proteins of the secretory pathway that include reticulocalbin, ERC-55, Cab45 and crocalbin. In order to further investigate the extracellular functions of calumenin we immobilized the recombinant protein to a column. After application of a placental tissue extract we were able to elute one protein that interacts with calumenin in the presence of Ca(2+). Amino acid sequencing identified this protein as serum amyloid P component (SAP). Furthermore, we verified and characterized the calumenin-SAP interaction by the surface plasmon resonance technique. The findings indicate that calumenin may participate in the immunological defense system and could be involved in the pathological process of amyloidosis that leads to formation of amyloid deposits seen in different types of tissues.

Pubmed ID: 10631319

Authors

  • Vorum H
  • Jacobsen C
  • HonorĂ© B

Journal

FEBS letters

Publication Data

January 14, 2000

Associated Grants

None

Mesh Terms

  • Amino Acid Sequence
  • Calcium-Binding Proteins
  • Chromatography, Affinity
  • Chromatography, Gel
  • Humans
  • Molecular Weight
  • Protein Binding
  • Serum Amyloid P-Component
  • Surface Plasmon Resonance