Preparing your results

Our searching services are busy right now. Your search will reload in five seconds.

X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Activated mutants of SHP-2 preferentially induce elongation of Xenopus animal caps.

http://www.ncbi.nlm.nih.gov/pubmed/10594032

In Xenopus ectodermal explants (animal caps), fibroblast growth factor (FGF) evokes two major events: induction of ventrolateral mesodermal tissues and elongation. The Xenopus FGF receptor (XFGFR) and certain downstream components of the XFGFR signal transduction pathway (e.g., members of the Ras/Raf/MEK/mitogen-activated protein kinase [MAPK] cascade) are required for both of these processes. Likewise, activated versions of these signaling components induce mesoderm and promote animal cap elongation. Previously, using a dominant negative mutant approach, we showed that the protein-tyrosine phosphatase SHP-2 is necessary for FGF-induced MAPK activation, mesoderm induction, and elongation of animal caps. Taking advantage of recent structural information, we now have generated novel, activated mutants of SHP-2. Here, we show that expression of these mutants induces animal cap elongation to an extent comparable to that evoked by FGF. Surprisingly, however, activated mutant-induced elongation can occur without mesodermal cytodifferentiation and is accompanied by minimal activation of the MAPK pathway and mesodermal marker expression. Our results implicate SHP-2 in a pathway(s) directing cell movements in vivo and identify potential downstream components of this pathway. Our activated mutants also may be useful for determining the specific functions of SHP-2 in other signaling systems.

Pubmed ID: 10594032 RIS Download

Mesh terms: Animals | Ectoderm | Fibroblast Growth Factors | Intracellular Signaling Peptides and Proteins | Mutation | Protein Conformation | Protein Tyrosine Phosphatase, Non-Receptor Type 11 | Protein Tyrosine Phosphatase, Non-Receptor Type 6 | Protein Tyrosine Phosphatases | Receptors, Fibroblast Growth Factor | SH2 Domain-Containing Protein Tyrosine Phosphatases | Signal Transduction | Xenopus laevis | src Homology Domains

Research resources used in this publication

None found

Research tools detected in this publication

None found

Data used in this publication

None found

Associated grants

  • Agency: NCI NIH HHS, Id: R01 CA49152
  • Agency: NIDDK NIH HHS, Id: R01 DK45943
  • Agency: NIDDK NIH HHS, Id: R01 DK51729

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.