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Identification of proteins that interact with NF-YA.

We used the yeast two-hybrid system to show that the serum response factor (SRF) and zinc-fingers and homeobox 1 (ZHXI) proteins interact with the A subunit of nuclear factor-Y (NF-YA). GST pulldown assays revealed that both proteins interact specifically with NF-YA in vitro. Amino acids located between 272 and 564, a region that contains two homeodomains, are required for the interaction of ZHX1 with NF-YA. Two different domains of NF-YA, a glutamine-rich region and a serine/threonine-rich region, are necessary for the interactions with ZHX1 and SRF, respectively.

Pubmed ID: 10571058

Authors

  • Yamada K
  • Osawa H
  • Granner DK

Journal

FEBS letters

Publication Data

October 22, 1999

Associated Grants

  • Agency: NCI NIH HHS, Id: CA68485
  • Agency: NIDDK NIH HHS, Id: DK20593
  • Agency: NIDDK NIH HHS, Id: DK46867

Mesh Terms

  • CCAAT-Binding Factor
  • DNA-Binding Proteins
  • Genes, Reporter
  • Homeodomain Proteins
  • Humans
  • Nuclear Proteins
  • Plasmids
  • Protein Binding
  • Serum Response Factor
  • Transcription Factors
  • Transcriptional Activation
  • Yeasts
  • Zinc Fingers