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Ggamma13 colocalizes with gustducin in taste receptor cells and mediates IP3 responses to bitter denatonium.

Gustducin is a transducin-like G protein selectively expressed in taste receptor cells. The alpha subunit of gustducin (alpha-gustducin) is critical for transduction of responses to bitter or sweet compounds. We identified a G-protein gamma subunit (Ggamma13) that colocalized with alpha-gustducin in taste receptor cells. Of 19 alpha-gustducin/Ggamma13-positive taste receptor cells profiled, all expressed the G protein beta3 subunit (Gbeta3); approximately 80% also expressed Gbeta1. Gustducin heterotrimers (alpha-gustducin/Gbeta1/Ggamma13) were activated by taste cell membranes plus bitter denatonium. Antibodies against Ggamma13 blocked the denatonium-induced increase of inositol trisphosphate (IP3) in taste tissue. We conclude that gustducin heterotrimers transduce responses to bitter and sweet compounds via alpha-gustducin's regulation of phosphodiesterase (PDE) and Gbetagamma's activation of phospholipase C (PLC).

Pubmed ID: 10570481

Authors

  • Huang L
  • Shanker YG
  • Dubauskaite J
  • Zheng JZ
  • Yan W
  • Rosenzweig S
  • Spielman AI
  • Max M
  • Margolskee RF

Journal

Nature neuroscience

Publication Data

December 18, 1999

Associated Grants

  • Agency: NIDCD NIH HHS, Id: DC03155
  • Agency: NIDCR NIH HHS, Id: DE10754
  • Agency: NIMH NIH HHS, Id: MH57241

Mesh Terms

  • Amino Acid Sequence
  • Animals
  • Cloning, Molecular
  • Enzyme Activation
  • Epithelial Cells
  • GTP-Binding Protein beta Subunits
  • GTP-Binding Protein gamma Subunits
  • GTP-Binding Proteins
  • Gene Expression Profiling
  • Heterotrimeric GTP-Binding Proteins
  • Humans
  • Inositol 1,4,5-Trisphosphate
  • Isoenzymes
  • Lingual Frenum
  • Mice
  • Mice, Transgenic
  • Molecular Sequence Data
  • Phospholipase C beta
  • Phosphoric Diester Hydrolases
  • Protein Binding
  • Quaternary Ammonium Compounds
  • RNA, Messenger
  • Sequence Alignment
  • Taste
  • Taste Buds
  • Transducin
  • Type C Phospholipases