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Ggamma13 colocalizes with gustducin in taste receptor cells and mediates IP3 responses to bitter denatonium.

Nature neuroscience | Dec 18, 1999

http://www.ncbi.nlm.nih.gov/pubmed/10570481

Gustducin is a transducin-like G protein selectively expressed in taste receptor cells. The alpha subunit of gustducin (alpha-gustducin) is critical for transduction of responses to bitter or sweet compounds. We identified a G-protein gamma subunit (Ggamma13) that colocalized with alpha-gustducin in taste receptor cells. Of 19 alpha-gustducin/Ggamma13-positive taste receptor cells profiled, all expressed the G protein beta3 subunit (Gbeta3); approximately 80% also expressed Gbeta1. Gustducin heterotrimers (alpha-gustducin/Gbeta1/Ggamma13) were activated by taste cell membranes plus bitter denatonium. Antibodies against Ggamma13 blocked the denatonium-induced increase of inositol trisphosphate (IP3) in taste tissue. We conclude that gustducin heterotrimers transduce responses to bitter and sweet compounds via alpha-gustducin's regulation of phosphodiesterase (PDE) and Gbetagamma's activation of phospholipase C (PLC).

Pubmed ID: 10570481 RIS Download

Mesh terms: Amino Acid Sequence | Animals | Cloning, Molecular | Enzyme Activation | Epithelial Cells | GTP-Binding Protein beta Subunits | GTP-Binding Protein gamma Subunits | GTP-Binding Proteins | Gene Expression Profiling | Heterotrimeric GTP-Binding Proteins | Humans | Inositol 1,4,5-Trisphosphate | Isoenzymes | Lingual Frenum | Mice | Mice, Transgenic | Molecular Sequence Data | Phospholipase C beta | Phosphoric Diester Hydrolases | Protein Binding | Quaternary Ammonium Compounds | RNA, Messenger | Sequence Alignment | Taste | Taste Buds | Transducin | Type C Phospholipases

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Associated grants

  • Agency: NIDCD NIH HHS, Id: DC03155
  • Agency: NIDCR NIH HHS, Id: DE10754
  • Agency: NIMH NIH HHS, Id: MH57241

GO (Data, Gene Annotation)

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