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Differential roles of TLR2 and TLR4 in recognition of gram-negative and gram-positive bacterial cell wall components.

Toll-like receptor (TLR) 2 and TLR4 are implicated in the recognition of various bacterial cell wall components, such as lipopolysaccharide (LPS). To investigate in vivo roles of TLR2, we generated TLR2-deficient mice. In contrast to LPS unresponsiveness in TLR4-deficient mice, TLR2-deficient mice responded to LPS to the same extent as wild-type mice. TLR2-deficient macrophages were hyporesponsive to several Gram-positive bacterial cell walls as well as Staphylococcus aureus peptidoglycan. TLR4-deficient macrophages lacked the response to Gram-positive lipoteichoic acids. These results demonstrate that TLR2 and TLR4 recognize different bacterial cell wall components in vivo and TLR2 plays a major role in Gram-positive bacterial recognition.

Pubmed ID: 10549626


  • Takeuchi O
  • Hoshino K
  • Kawai T
  • Sanjo H
  • Takada H
  • Ogawa T
  • Takeda K
  • Akira S



Publication Data

October 23, 1999

Associated Grants


Mesh Terms

  • Animals
  • Antigens, Bacterial
  • Cell Wall
  • Corynebacterium diphtheriae
  • Drosophila Proteins
  • Escherichia coli
  • Female
  • Gram-Negative Bacteria
  • Gram-Positive Bacteria
  • Interleukin-1 Receptor-Associated Kinases
  • Lipid A
  • Lipopolysaccharides
  • Macrophages, Peritoneal
  • Male
  • Membrane Glycoproteins
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • NF-kappa B
  • Nocardia
  • Peptidoglycan
  • Protein Isoforms
  • Protein Kinases
  • Receptors, Cell Surface
  • Salmonella
  • Signal Transduction
  • Species Specificity
  • Staphylococcus aureus
  • Teichoic Acids
  • Toll-Like Receptor 2
  • Toll-Like Receptor 4
  • Toll-Like Receptors