We have updated our privacy policy. If you have any question, contact us at privacy@scicrunch.org. Dismiss and don't show again

Searching across hundreds of databases

Our searching services are busy right now. Your search will reload in five seconds.

Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Identification of human APC10/Doc1 as a subunit of anaphase promoting complex.

Oncogene | Sep 16, 1999

Anaphase-promoting complex or cyclosome (APC) is a ubiquitin ligase which specifically targets mitotic regulatory factors such as Pds1/Cut2 and cyclin B. Identification of the subunits of multiprotein complex APC in several species revealed the highly conserved composition of APC from yeast to human. It has been reported, however, that vertebrate APC is composed of at least eight subunits, APC1 to APC8, while budding yeast APC is constituted of at least 12 components, Apc1 to Apc13. It has not yet been clearly understood whether additional components found in budding yeast, Apc9 to Apc13, are actually composed of mammalian APC. Here we isolated and characterized human APC10/Doc1, and found that APC10/Doc1 binds to APC core subunits throughout the cell cycle. Further, it was found that APC10/Doc1 is localized in centrosomes and mitotic spindles throughout mitosis, while it is also localized in kinetochores from prophase to anaphase and in midbody in telophase and cytokinesis. These results strongly support the notion that human APC10/Doc1 may be one of the APC core subunits rather than the transiently associated regulatory factor.

Pubmed ID: 10498862 RIS Download

Mesh terms: Amino Acid Sequence | Anaphase | Anaphase-Promoting Complex-Cyclosome | Apc1 Subunit, Anaphase-Promoting Complex-Cyclosome | Apc10 Subunit, Anaphase-Promoting Complex-Cyclosome | Cell Cycle | Cell Cycle Proteins | Centromere | Centrosome | DNA, Complementary | Fungal Proteins | HeLa Cells | Humans | Ligases | Molecular Sequence Data | Multienzyme Complexes | Saccharomyces cerevisiae | Saccharomyces cerevisiae Proteins | Sequence Alignment | Sequence Homology, Amino Acid | Species Specificity | Spindle Apparatus | Ubiquitin-Protein Ligase Complexes | Ubiquitin-Protein Ligases

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.

We have not found any resources mentioned in this publication.