Gene targeting restricted to mouse striated muscle lineage.
Spatially and temporally regulated somatic mutations can be achieved by using the Cre/LoxP recombination system of bacteriophage P1. In order to develop gene knockouts restricted to striated muscle, we generated a transgenic mouse line expressing Cre recombinase under the control of the human alpha-skeletal actin promoter. Specific excision of a loxP-flanked gene was demonstrated in striated muscle, heart and skeletal muscle, in a pattern very similar to the expression of the endogenous alpha-skeletal actin gene. Therefore, the reported transgenic line can be used to target inactivation or activation of a given gene to the skeletal muscle lineage.
Pubmed ID: 10481039 RIS Download
Actins | Animals | Chickens | Gene Targeting | Humans | Integrases | Lac Operon | Mice | Mice, Inbred C57BL | Mice, Knockout | Muscle, Skeletal | Myocardium | Promoter Regions, Genetic | Viral Proteins