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Gene targeting restricted to mouse striated muscle lineage.

Nucleic acids research | Oct 1, 1999

http://www.ncbi.nlm.nih.gov/pubmed/10481039

Spatially and temporally regulated somatic mutations can be achieved by using the Cre/LoxP recombination system of bacteriophage P1. In order to develop gene knockouts restricted to striated muscle, we generated a transgenic mouse line expressing Cre recombinase under the control of the human alpha-skeletal actin promoter. Specific excision of a loxP-flanked gene was demonstrated in striated muscle, heart and skeletal muscle, in a pattern very similar to the expression of the endogenous alpha-skeletal actin gene. Therefore, the reported transgenic line can be used to target inactivation or activation of a given gene to the skeletal muscle lineage.

Pubmed ID: 10481039 RIS Download

Mesh terms: Actins | Animals | Chickens | Gene Targeting | Humans | Integrases | Lac Operon | Mice | Mice, Inbred C57BL | Mice, Knockout | Muscle, Skeletal | Myocardium | Promoter Regions, Genetic | Viral Proteins