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Gene targeting restricted to mouse striated muscle lineage.

Spatially and temporally regulated somatic mutations can be achieved by using the Cre/LoxP recombination system of bacteriophage P1. In order to develop gene knockouts restricted to striated muscle, we generated a transgenic mouse line expressing Cre recombinase under the control of the human alpha-skeletal actin promoter. Specific excision of a loxP-flanked gene was demonstrated in striated muscle, heart and skeletal muscle, in a pattern very similar to the expression of the endogenous alpha-skeletal actin gene. Therefore, the reported transgenic line can be used to target inactivation or activation of a given gene to the skeletal muscle lineage.

Pubmed ID: 10481039

Authors

  • Miniou P
  • Tiziano D
  • Frugier T
  • Roblot N
  • Le Meur M
  • Melki J

Journal

Nucleic acids research

Publication Data

October 1, 1999

Associated Grants

None

Mesh Terms

  • Actins
  • Animals
  • Chickens
  • Gene Targeting
  • Humans
  • Integrases
  • Lac Operon
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Muscle, Skeletal
  • Myocardium
  • Promoter Regions, Genetic
  • Viral Proteins