Preparing your results

Our searching services are busy right now. Your search will reload in five seconds.

X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Requirement of Rsk-2 for epidermal growth factor-activated phosphorylation of histone H3.

Science (New York, N.Y.) | Aug 6, 1999

http://www.ncbi.nlm.nih.gov/pubmed/10436156

During the immediate-early response of mammalian cells to mitogens, histone H3 is rapidly and transiently phosphorylated by one or more unidentified kinases. Rsk-2, a member of the pp90rsk family of kinases implicated in growth control, was required for epidermal growth factor (EGF)-stimulated phosphorylation of H3. RSK-2 mutations in humans are linked to Coffin-Lowry syndrome (CLS). Fibroblasts derived from a CLS patient failed to exhibit EGF-stimulated phosphorylation of H3, although H3 was phosphorylated during mitosis. Introduction of the wild-type RSK-2 gene restored EGF-stimulated phosphorylation of H3 in CLS cells. In addition, disruption of the RSK-2 gene by homologous recombination in murine embryonic stem cells abolished EGF-stimulated phosphorylation of H3. H3 appears to be a direct or indirect target of Rsk-2, suggesting that chromatin remodeling might contribute to mitogen-activated protein kinase-regulated gene expression.

Pubmed ID: 10436156 RIS Download

Mesh terms: 3T3 Cells | Abnormalities, Multiple | Animals | Calcium-Calmodulin-Dependent Protein Kinases | Cell Line, Transformed | Cell Nucleus | Cells, Cultured | Epidermal Growth Factor | Gene Expression Regulation | Gene Targeting | Histones | Humans | Mice | Mitosis | Mutation | Phosphorylation | Ribosomal Protein S6 Kinases | Signal Transduction | Stem Cells | Syndrome

Research resources used in this publication

None found

Research tools detected in this publication

None found

Data used in this publication

None found

Associated grants

  • Agency: NIGMS NIH HHS, Id: GM40922

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.