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Isolation and characterization of ARA160 as the first androgen receptor N-terminal-associated coactivator in human prostate cells.

The androgen receptor (AR) is a member of the steroid receptor superfamily that may require coactivators for proper or maximal transactivation. Using a purified AR N-terminal peptide as a probe to screen the human testis expression library, we identified an androgen-enhanced AR N-terminal-associated protein ARA160, which consists of 1,093 amino acids with an apparent molecular mass of 160 kDa. Sequence comparison in GenBank(TM) reveals that ARA160 shares an identical sequence with a HIV-1 TATA element modulatory factor, TMF. The far-Western blotting and co-immunoprecipitation assays demonstrate that the AR can interact directly with ARA160/TMF. Affinity gel pull-down and mammalian two-hybrid assays further suggest androgen can enhance significantly the interaction between AR and ARA160. Transient transfection assays demonstrated that ARA160 might function as a coactivator for AR-mediated transactivation in human prostate cancer PC-3 cells. Our data further suggest that this AR N-terminal coactivator can function cooperatively with AR C-terminal coactivator, ARA70, in PC-3 cells. Together, our data demonstrate that ARA160 might represent the first identified androgen-enhanced N-terminal coactivator for the AR.

Pubmed ID: 10428808


  • Hsiao PW
  • Chang C


The Journal of biological chemistry

Publication Data

August 6, 1999

Associated Grants

  • Agency: NCI NIH HHS, Id: CA55639
  • Agency: NCI NIH HHS, Id: CA68518
  • Agency: NCI NIH HHS, Id: CA71570

Mesh Terms

  • Amino Acid Sequence
  • Androgens
  • Cloning, Molecular
  • DNA-Binding Proteins
  • Humans
  • Ligands
  • Male
  • Mammary Tumor Virus, Mouse
  • Molecular Sequence Data
  • Nuclear Receptor Coactivators
  • Oncogene Proteins
  • Promoter Regions, Genetic
  • Prostate
  • Prostate-Specific Antigen
  • Protein Binding
  • Receptors, Androgen
  • Receptors, Estrogen
  • Receptors, Glucocorticoid
  • Response Elements
  • Sequence Analysis, DNA
  • Terminal Repeat Sequences
  • Trans-Activators
  • Transcription Factors
  • Transcriptional Activation