Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

An adipogenic cofactor bound by the differentiation domain of PPARgamma.

Ligand activation of the nuclear receptor PPARgamma induces adipogenesis and increases insulin sensitivity, while activation of other PPAR isoforms (-alpha and -delta) induces little or no fat cell differentiation. Expression and activation of chimeras formed between PPARgamma and PPARdelta in fibroblasts has allowed us to localize a major domain of PPARgamma responsible for adipogenesis to the N-terminal 138 amino acids, a region with AF-1 transcriptional activity. Using this region of PPARgamma as bait, we have used a yeast two-hybrid screen to clone a novel protein, termed PGC-2, containing a partial SCAN domain. PGC-2 binds to and increases the transcriptional activity of PPARgamma but does not interact with other PPARs or most other nuclear receptors. Ectopic expression of PGC-2 in preadipocytes containing endogenous PPARgamma causes a dramatic increase in fat cell differentiation at both the morphological and molecular levels. These results suggest that interactions between PGC-2, a receptor isoform-selective cofactor and PPARgamma contribute to the adipogenic action of this receptor.

Pubmed ID: 10393183


  • Castillo G
  • Brun RP
  • Rosenfield JK
  • Hauser S
  • Park CW
  • Troy AE
  • Wright ME
  • Spiegelman BM


The EMBO journal

Publication Data

July 1, 1999

Associated Grants

  • Agency: NIDDK NIH HHS, Id: DK-09090
  • Agency: NIDDK NIH HHS, Id: R01 5R37DK31405

Mesh Terms

  • Adipocytes
  • Amino Acid Sequence
  • Animals
  • Azo Compounds
  • Base Sequence
  • Cell Differentiation
  • Cell Line
  • Cloning, Molecular
  • Gene Expression Regulation
  • Mice
  • Molecular Sequence Data
  • Protein Binding
  • Protein Isoforms
  • RNA, Messenger
  • Receptors, Cytoplasmic and Nuclear
  • Receptors, Estrogen
  • Recombinant Fusion Proteins
  • Stem Cells
  • Substrate Specificity
  • Transcription Factors
  • Transcription, Genetic
  • Transfection
  • Yeasts