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Tip60 is a nuclear hormone receptor coactivator.

The androgen receptor (AR) is a member of the nuclear hormone receptor superfamily. Recent work in this field has been focused upon defining the mechanisms of transcriptional control exacted by members of this superfamily. Using a COOH-terminal region of the human AR in a yeast two-hybrid screen, we have identified Tip60 as an AR-interacting protein. In this report, we show that Tip60, which was originally identified as a coactivator for the human immunodeficiency virus TAT protein, can enhance AR-mediated transactivation in a ligand-dependent manner in LNCaP and COS-1 cell lines. In addition, our experiments show that Tip60 can also enhance transactivation through the estrogen receptor and progesterone receptor in a ligand-dependent manner; thus identifying Tip60 as a nuclear hormone receptor coactivator. Our studies also demonstrate that Tip60 co-immunoprecipitates with the full-length AR in vitro and that, in our system, Tip60 enhances transactivation to levels observed with the coactivators steroid receptor coactivator 1, p300, and CREB-binding protein. The importance of such proteins in enhancing nuclear hormone receptor-mediated transcriptional activation is widely accepted, and this work suggests that Tip60 may have an equally important role to play.

Pubmed ID: 10364196


  • Brady ME
  • Ozanne DM
  • Gaughan L
  • Waite I
  • Cook S
  • Neal DE
  • Robson CN


The Journal of biological chemistry

Publication Data

June 18, 1999

Associated Grants


Mesh Terms

  • Acetyltransferases
  • Animals
  • CREB-Binding Protein
  • Cell Cycle Proteins
  • Cell Line
  • Histone Acetyltransferases
  • Humans
  • Nuclear Proteins
  • Nuclear Receptor Coactivator 1
  • Proteins
  • Receptors, Androgen
  • Receptors, Cytoplasmic and Nuclear
  • Receptors, Progesterone
  • Trans-Activators
  • Transcription Factors
  • Transcriptional Activation
  • Transfection
  • Yeasts
  • p300-CBP Transcription Factors