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Structure of the nuclear transport complex karyopherin-beta2-Ran x GppNHp.

Nature | May 20, 1999

Transport factors in the karyopherin-beta (also called importin-beta) family mediate the movement of macromolecules in nuclear-cytoplasmic transport pathways. Karyopherin-beta2 (transportin) binds a cognate import substrate and targets it to the nuclear pore complex. In the nucleus, Ran x GTP binds karyopherin-beta2 and dissociates the substrate. Here we present the 3.0 A structure of the karyopherin-beta2-Ran x GppNHp complex where GppNHp is a non-hydrolysable GTP analogue. Karyopherin-beta2 contains eighteen HEAT repeats arranged into two continuous orthogonal arches. Ran is clamped in the amino-terminal arch and substrate-binding activity is mapped to the carboxy-terminal arch. A large loop in HEAT repeat 7 spans both arches. Interactions of the loop with Ran and the C-terminal arch implicate it in GTPase-mediated dissociation of the import-substrate. Ran x GppNHp in the complex shows extensive structural rearrangement, compared to Ran GDP, in regions contacting karyopherin-beta2. This provides a structural basis for the specificity of the karyopherin-beta family for the GTP-bound state of Ran, as well as a rationale for interactions of the karyopherin-Ran complex with the regulatory proteins ranGAP, ranGEF and ranBP1.

Pubmed ID: 10353245 RIS Download

Mesh terms: Amino Acid Sequence | Biological Transport | Cell Nucleus | Crystallography, X-Ray | Escherichia coli | Guanosine Triphosphate | Guanylyl Imidodiphosphate | Humans | Karyopherins | Macromolecular Substances | Models, Molecular | Molecular Sequence Data | Nuclear Proteins | Protein Conformation | Receptors, Cytoplasmic and Nuclear | Recombinant Proteins | Repetitive Sequences, Amino Acid | ran GTP-Binding Protein