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Recruitment of a ROC1-CUL1 ubiquitin ligase by Skp1 and HOS to catalyze the ubiquitination of I kappa B alpha.

Molecular cell | Apr 27, 1999

http://www.ncbi.nlm.nih.gov/pubmed/10230406

Activation of the transcription factor NF-kappa B in response to proinflammatory stimuli requires the phosphorylation-triggered and ubiquitin-dependent degradation of the NF-kappa B inhibitor, I kappa B alpha. Here, we show the in vitro reconstitution of the phosphorylation-dependent ubiquitination of I kappa B alpha with purified components. ROC1, a novel SCF-associated protein, is recruited by cullin 1 to form a quatemary SCFHOS-ROC1 holenzyme (with Skp1 and the beta-TRCP homolog HOS). SCFHOS-ROC1 binds IKK beta-phosphorylated I kappa B alpha and catalyzes its ubiquitination in the presence of ubiquitin, E1, and Cdc34. ROC1 plays a unique role in the ubiquitination reaction by heterodimerizing with cullin 1 to catalyze ubiquitin polymerization.

Pubmed ID: 10230406 RIS Download

Mesh terms: Amino Acid Sequence | Cell Cycle Proteins | Cullin Proteins | DNA-Binding Proteins | HeLa Cells | Humans | I-kappa B Kinase | I-kappa B Proteins | Molecular Sequence Data | Peptide Synthases | Phosphorylation | Protein-Serine-Threonine Kinases | S-Phase Kinase-Associated Proteins | SKP Cullin F-Box Protein Ligases | Ubiquitins

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Associated grants

  • Agency: NCI NIH HHS, Id: CA78419
  • Agency: NIGMS NIH HHS, Id: GM55059

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