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Nectin/PRR: an immunoglobulin-like cell adhesion molecule recruited to cadherin-based adherens junctions through interaction with Afadin, a PDZ domain-containing protein.

We have isolated a novel actin filament-binding protein, named afadin, localized at cadherin-based cell-cell adherens junctions (AJs) in various tissues and cell lines. Afadin has one PDZ domain, three proline-rich regions, and one actin filament-binding domain. We found here that afadin directly interacted with a family of the immunoglobulin superfamily, which was isolated originally as the poliovirus receptor-related protein (PRR) family consisting of PRR1 and -2, and has been identified recently to be the alphaherpes virus receptor. PRR has a COOH-terminal consensus motif to which the PDZ domain of afadin binds. PRR and afadin were colocalized at cadherin-based cell-cell AJs in various tissues and cell lines. In E-cadherin-expressing EL cells, PRR was recruited to cadherin-based cell-cell AJs through interaction with afadin. PRR showed Ca2+-independent cell-cell adhesion activity. These results indicate that PRR is a cell-cell adhesion molecule of the immunoglobulin superfamily which is recruited to cadherin-based cell-cell AJs through interaction with afadin. We rename PRR as nectin (taken from the Latin word "necto" meaning "to connect").

Pubmed ID: 10225955


  • Takahashi K
  • Nakanishi H
  • Miyahara M
  • Mandai K
  • Satoh K
  • Satoh A
  • Nishioka H
  • Aoki J
  • Nomoto A
  • Mizoguchi A
  • Takai Y


The Journal of cell biology

Publication Data

May 3, 1999

Associated Grants


Mesh Terms

  • Alternative Splicing
  • Amino Acid Sequence
  • Animals
  • COS Cells
  • Cadherins
  • Calcium
  • Cell Adhesion Molecules
  • Cell Aggregation
  • Epithelial Cells
  • Intercellular Junctions
  • Kinesin
  • Membrane Glycoproteins
  • Mice
  • Microfilament Proteins
  • Microscopy, Electron
  • Myocardium
  • Myosins
  • Protein Structure, Tertiary
  • Rabbits
  • Receptors, Tumor Necrosis Factor
  • Receptors, Tumor Necrosis Factor, Member 14
  • Receptors, Virus
  • Vinculin