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Rbx1, a component of the VHL tumor suppressor complex and SCF ubiquitin ligase.

The von Hippel-Lindau (VHL) tumor suppressor gene is mutated in most human kidney cancers. The VHL protein is part of a complex that includes Elongin B, Elongin C, and Cullin-2, proteins associated with transcriptional elongation and ubiquitination. Here it is shown that the endogenous VHL complex in rat liver also includes Rbx1, an evolutionarily conserved protein that contains a RING-H2 fingerlike motif and that interacts with Cullins. The yeast homolog of Rbx1 is a subunit and potent activator of the Cdc53-containing SCFCdc4 ubiquitin ligase required for ubiquitination of the cyclin-dependent kinase inhibitor Sic1 and for the G1 to S cell cycle transition. These findings provide a further link between VHL and the cellular ubiquitination machinery.

Pubmed ID: 10213691

Authors

  • Kamura T
  • Koepp DM
  • Conrad MN
  • Skowyra D
  • Moreland RJ
  • Iliopoulos O
  • Lane WS
  • Kaelin WG
  • Elledge SJ
  • Conaway RC
  • Harper JW
  • Conaway JW

Journal

Science (New York, N.Y.)

Publication Data

April 23, 1999

Associated Grants

  • Agency: NIA NIH HHS, Id: AG-11085
  • Agency: NIGMS NIH HHS, Id: GM41628

Mesh Terms

  • Amino Acid Sequence
  • Animals
  • Carrier Proteins
  • Cell Cycle
  • Cell Cycle Proteins
  • Cell Line
  • Cullin Proteins
  • Cyclin-Dependent Kinase Inhibitor Proteins
  • F-Box Proteins
  • Fungal Proteins
  • Ligases
  • Liver
  • Male
  • Molecular Sequence Data
  • Peptide Synthases
  • Proteins
  • Rats
  • Rats, Sprague-Dawley
  • Recombinant Fusion Proteins
  • S-Phase Kinase-Associated Proteins
  • SKP Cullin F-Box Protein Ligases
  • Saccharomyces cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Sequence Alignment
  • Transcription Factors
  • Tumor Suppressor Proteins
  • Ubiquitin-Protein Ligases
  • Ubiquitins
  • Von Hippel-Lindau Tumor Suppressor Protein