Searching across hundreds of databases

Our searching services are busy right now. Your search will reload in five seconds.

Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Control of mRNA decay by heat shock-ubiquitin-proteasome pathway.

Science (New York, N.Y.) | Apr 16, 1999

Cytokine and proto-oncogene messenger RNAs (mRNAs) are rapidly degraded through AU-rich elements in the 3' untranslated region. Rapid decay involves AU-rich binding protein AUF1, which complexes with heat shock proteins hsc70-hsp70, translation initiation factor eIF4G, and poly(A) binding protein. AU-rich mRNA decay is associated with displacement of eIF4G from AUF1, ubiquitination of AUF1, and degradation of AUF1 by proteasomes. Induction of hsp70 by heat shock, down-regulation of the ubiquitin-proteasome network, or inactivation of ubiquitinating enzyme E1 all result in hsp70 sequestration of AUF1 in the perinucleus-nucleus, and all three processes block decay of AU-rich mRNAs and AUF1 protein. These results link the rapid degradation of cytokine mRNAs to the ubiquitin-proteasome pathway.

Pubmed ID: 10205060 RIS Download

Mesh terms: 3' Untranslated Regions | Carrier Proteins | Cell Nucleus | Cysteine Endopeptidases | Cysteine Proteinase Inhibitors | Cytoplasm | Eukaryotic Initiation Factor-4G | Granulocyte-Macrophage Colony-Stimulating Factor | HSC70 Heat-Shock Proteins | HSP70 Heat-Shock Proteins | HeLa Cells | Heat-Shock Response | Heterogeneous-Nuclear Ribonucleoprotein D | Humans | Leupeptins | Multienzyme Complexes | Peptide Initiation Factors | Poly(A)-Binding Proteins | Proteasome Endopeptidase Complex | Protein Binding | RNA, Messenger | RNA-Binding Proteins | Transfection | Ubiquitins

Research resources used in this publication

None found

Research tools detected in this publication

None found

Data used in this publication

None found

Associated grants

  • Agency: NCI NIH HHS, Id: CA42357
  • Agency: NCI NIH HHS, Id: CA52443

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.

We have not found any resources mentioned in this publication.