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Reconstitution of a core chromatin remodeling complex from SWI/SNF subunits.

Molecular cell | Feb 13, 1999

http://www.ncbi.nlm.nih.gov/pubmed/10078207

Protein complexes of the SWI/SNF family remodel nucleosome structure in an ATP-dependent manner. Each complex contains between 8 and 15 subunits, several of which are highly conserved between yeast, Drosophila, and humans. We have reconstituted an ATP-dependent chromatin remodeling complex using a subset of conserved subunits. Unexpectedly, both BRG1 and hBRM, the ATPase subunits of human SWI/SNF complexes, are capable of remodeling mono-nucleosomes and nucleosomal arrays as purified proteins. The addition of INI1, BAF155, and BAF170 to BRG1 increases remodeling activity to a level comparable to that of the whole hSWI/SNF complex. These data define the functional core of the hSWI/SNF complex.

Pubmed ID: 10078207 RIS Download

Mesh terms: Actins | Adenosine Triphosphate | Cell-Free System | Chromatin | Chromosomal Proteins, Non-Histone | DNA Helicases | DNA-Binding Proteins | Drosophila Proteins | Evolution, Molecular | Humans | Macromolecular Substances | Nuclear Proteins | Nucleosomes | RNA-Binding Proteins | Recombinant Fusion Proteins | Ribonucleoprotein, U1 Small Nuclear | Transcription Factors

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