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Reconstitution of a core chromatin remodeling complex from SWI/SNF subunits.

Protein complexes of the SWI/SNF family remodel nucleosome structure in an ATP-dependent manner. Each complex contains between 8 and 15 subunits, several of which are highly conserved between yeast, Drosophila, and humans. We have reconstituted an ATP-dependent chromatin remodeling complex using a subset of conserved subunits. Unexpectedly, both BRG1 and hBRM, the ATPase subunits of human SWI/SNF complexes, are capable of remodeling mono-nucleosomes and nucleosomal arrays as purified proteins. The addition of INI1, BAF155, and BAF170 to BRG1 increases remodeling activity to a level comparable to that of the whole hSWI/SNF complex. These data define the functional core of the hSWI/SNF complex.

Pubmed ID: 10078207

Authors

  • Phelan ML
  • Sif S
  • Narlikar GJ
  • Kingston RE

Journal

Molecular cell

Publication Data

February 13, 1999

Associated Grants

None

Mesh Terms

  • Actins
  • Adenosine Triphosphate
  • Cell-Free System
  • Chromatin
  • Chromosomal Proteins, Non-Histone
  • DNA Helicases
  • DNA-Binding Proteins
  • Drosophila Proteins
  • Evolution, Molecular
  • Humans
  • Macromolecular Substances
  • Nuclear Proteins
  • Nucleosomes
  • RNA-Binding Proteins
  • Recombinant Fusion Proteins
  • Ribonucleoprotein, U1 Small Nuclear
  • Transcription Factors