Reconstitution of a core chromatin remodeling complex from SWI/SNF subunits.
Protein complexes of the SWI/SNF family remodel nucleosome structure in an ATP-dependent manner. Each complex contains between 8 and 15 subunits, several of which are highly conserved between yeast, Drosophila, and humans. We have reconstituted an ATP-dependent chromatin remodeling complex using a subset of conserved subunits. Unexpectedly, both BRG1 and hBRM, the ATPase subunits of human SWI/SNF complexes, are capable of remodeling mono-nucleosomes and nucleosomal arrays as purified proteins. The addition of INI1, BAF155, and BAF170 to BRG1 increases remodeling activity to a level comparable to that of the whole hSWI/SNF complex. These data define the functional core of the hSWI/SNF complex.
Pubmed ID: 10078207 RIS Download
Actins | Adenosine Triphosphate | Cell-Free System | Chromatin | Chromosomal Proteins, Non-Histone | DNA Helicases | DNA-Binding Proteins | Drosophila Proteins | Evolution, Molecular | Humans | Macromolecular Substances | Nuclear Proteins | Nucleosomes | RNA-Binding Proteins | Recombinant Fusion Proteins | Ribonucleoprotein, U1 Small Nuclear | Transcription Factors