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Identification of a human HECT family protein with homology to the Drosophila tumor suppressor gene hyperplastic discs.

Use of the differential display technique to isolate progestin-regulated genes in T-47D human breast cancer cells led to identification of a novel gene, EDD. The cDNA sequence contains a 2799 amino acid open reading frame sharing 40% identity with the predicted 2894 amino acid product of the Drosophila melanogaster tumor suppressor gene hyperplastic discs, while the carboxy-terminal 889 amino acids show 96% identity to a rat 100 kDa HECT domain protein. EDD mRNA was progestin-induced in T-47D cells and was highly abundant in testes and expressed at moderately high levels in other tissues, suggesting a broad role for EDD. Anti-EDD antibodies immunoprecipitated an approximately 300 kDa protein from T-47D cell lysates. HECT family proteins function as E3 ubiquitin-protein ligases, targeting specific proteins for ubiquitin-mediated proteolysis. EDD is likely to function as an E3 as in vitro translated protein bound ubiquitin reversibly through a conserved HECT domain cysteine residue. EDD was localized by FISH to chromosome 8q22, a locus disrupted in a variety of cancers. Given the homology between EDD and the hyperplastic discs protein, which is required for control of imaginal disc growth in Drosophila, EDD potentially has a role in regulation of cell proliferation or differentiation.

Pubmed ID: 10030672


  • Callaghan MJ
  • Russell AJ
  • Woollatt E
  • Sutherland GR
  • Sutherland RL
  • Watts CK



Publication Data

December 31, 1998

Associated Grants


Mesh Terms

  • Amino Acid Sequence
  • Animals
  • Blotting, Northern
  • Chromosomes, Human, Pair 8
  • Cloning, Molecular
  • Drosophila melanogaster
  • Genetic Techniques
  • Helminth Proteins
  • Humans
  • Ligases
  • Male
  • Molecular Sequence Data
  • Peptide Synthases
  • Pregnenediones
  • Proteins
  • Rats
  • Sequence Homology, Amino Acid
  • Ubiquitin-Protein Ligases
  • Ubiquitins