Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Early embryonic death of mice deficient in gamma-adaptin.

Intracellular protein transport and sorting by vesicles in the secretory and endocytic pathways requires the formation of a protein coat on the membrane. The heterotetrameric adaptor protein complex 1 (AP-1) promotes the formation of clathrin-coated vesicles at the trans-Golgi network. AP-1 interacts with various sorting signals in the cytoplasmic tails of cargo molecules, thus indicating a function in protein sorting. We generated mutants of the gamma-adaptin subunit of AP-1 in mice to investigate its role in post-Golgi vesicle transport and sorting processes. gamma-Adaptin-deficient embryos develop until day 3.5 post coitus and die during the prenidation period, revealing that AP-1 is essential for viability. In heterozygous mice the amount of AP-1 complexes is reduced to half of controls. Free beta1- or micro1 chains were not detectable, indicating that they are unstable unless they are part of AP-1 complexes. Heterozygous mice weigh less then their wild-type littermates and show impaired T cell development.

Pubmed ID: 10026148


  • Zizioli D
  • Meyer C
  • Guhde G
  • Saftig P
  • von Figura K
  • Schu P


The Journal of biological chemistry

Publication Data

February 26, 1999

Associated Grants


Mesh Terms

  • Adaptor Protein Complex gamma Subunits
  • Animals
  • Fetal Death
  • Genes, Lethal
  • Heterozygote
  • Membrane Proteins
  • Mice
  • Mutagenesis, Site-Directed
  • Phenotype