• Register
X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

X

Leaving Community

Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.

No
Yes

Elongator, a multisubunit component of a novel RNA polymerase II holoenzyme for transcriptional elongation.

The form of RNA polymerase II (RNAPII) engaged in transcriptional elongation was isolated. Elongating RNAPII was associated with a novel multisubunit complex, termed elongator, whose stable interaction was dependent on a hyperphosphorylated state of the RNAPII carboxy-terminal domain (CTD). A free form of elongator was also isolated, demonstrating the discrete nature of the complex, and free elongator could bind directly to RNAPII. The gene encoding the largest subunit of elongator, ELP1, was cloned. Phenotypes of yeast elp1 delta cells demonstrated an involvement of elongator in transcriptional elongation as well as activation in vivo. Our data indicate that the transition from transcriptional initiation to elongation involves an exchange of the multiprotein mediator complex for elongator in a reaction coupled to CTD hyperphosphorylation.

Pubmed ID: 10024884

Authors

  • Otero G
  • Fellows J
  • Li Y
  • de Bizemont T
  • Dirac AM
  • Gustafsson CM
  • Erdjument-Bromage H
  • Tempst P
  • Svejstrup JQ

Journal

Molecular cell

Publication Data

January 12, 1999

Associated Grants

  • Agency: NCI NIH HHS, Id: P30 CA08748

Mesh Terms

  • Cell Division
  • Cloning, Molecular
  • Fungal Proteins
  • Gene Expression Regulation
  • Phenotype
  • Phosphorylation
  • Protein Binding
  • RNA Polymerase II
  • Saccharomyces cerevisiae
  • Transcription Factors
  • Transcription Factors, General
  • Transcription, Genetic
  • Transcriptional Activation
  • Transcriptional Elongation Factors