URL: http://antibodyregistry.org/AB_2744545
Proper Citation: (Thomas Shenk; Princeton University Cat# 7H1-5, RRID:AB_2744545)
Target Antigen: pUL26
Host Organism: mouse
Clonality: monoclonal
Comments: "To produce a UL26-specific monoclonal antibody, the UL26-Flag DNA segment from pBlueUL26F was subcloned into pGex4T1 to create pGex26F. A glutathione S-transferase (GST) fusion protein, GST-26F, was then produced by transfection of Escherichia coli BL-21 with pGex26F. The resulting fusion protein was affinity purified on glutathione Sepharose as recommended by the manufacturer (Amersham Pharmacia Biotech). Purified protein was used to immunize 5-week-old BALB/c mice. Mice were boosted with GST-26F twice and screened for seroconversion, and spleens were harvested for fusion. Hybridomas secreting antibody to pUL26 were screened, selected, and amplified."
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Source: Antibody Registry